Document Type

Paper- Restricted to Campus Access

Publication Date

4-29-2026

Faculty Mentor

Stephen C. Kolwicz Jr.

Abstract

The study of mitochondrial respiration is crucial for understanding fundamental differences across tissues such as cardiac, hepatic, and skeletal tissues in mice. Moreover, mitochondrial respiration data can be used to analyze differences between control mice and treatment groups. This study aimed to comprehensively characterize mitochondrial respiration, citrate synthase activity, and protein yields across three metabolically distinct mouse tissues to understand mitochondrial processes better. Mitochondria were isolated from the hearts, livers, and quadriceps of C57BL/6NCrl mice using differential centrifugation. Oxygen consumption was measured via a Clark-type electrode under State 2 (substrate-supported) and State 3 (ADP-stimulated) conditions. Citrate synthase activity was quantified spectrophotometrically as a marker of mitochondrial content. Protein concentrations were determined by BCA assay to normalize all measurements. These data will establish a comprehensive reference dataset for mouse mitochondrial function across key metabolic tissues, demonstrating that mitochondrial oxidative capacity scales with physiological energy demands. This resource provides a baseline for future studies investigating mitochondrial aspects in our research.

Comments

Presented as part of the Ursinus College Celebration of Student Achievement (CoSA) held April 29, 2026.

The downloadable file is a PowerPoint poster.

Download

Restricted

Available to Ursinus community only.

Share

COinS