Submission Date
4-24-2023
Document Type
Paper- Restricted to Campus Access
Department
Neuroscience
Adviser
Jennifer King
Committee Member
Samantha Wilner
Committee Member
Lauren Makuch
Department Chair
Carlita Favero
Project Description
As the primary macrophage of the central nervous system, microglia play a significant role in the immune response of the brain. Studying microglia dysfunction is important for understanding neurodegenerative disease pathology, such as HIV-associated neurocognitive disorder (HAND). Immortalized cell lines or primary microglia from neonatal mice are the most commonly used and accessible in vitro microglia models because isolating and culturing cells from adult mice is much more difficult, timely, and costly. However, because of their natural ageing, primary adult microglia are a much more physiologically adequate model for neurodegenerative diseases. The aim of this project is to establish a method for successfully isolating these primary microglia from adult mice to be used in further studies examining their neuroinflammatory response and its role in neurodegeneration.
Recommended Citation
Rodriguez, Carly, "Isolation of Primary Microglia From Adult Mice to Examine Neuroinflammatory Response in Neurodegenerative Disease Modelling" (2023). Neuroscience Honors Papers. 19.
https://digitalcommons.ursinus.edu/neuro_hon/19
