Influence of Wnt Proteins Following Acute Skeletal Muscle Injury in Mice and Freezing Protocols for Skeletal Muscle Histology

Author

Samantha Keoonela, Ursinus CollegeFollow
Bradley Garcia, Ursinus CollegeFollow

Submission Date

7-22-2016

Document Type

Paper- Restricted to Campus Access

Department

Health & Exercise Physiology

Faculty Mentor

April Carpenter

Comments

Presented during the 18th Annual Summer Fellows Symposium, July 22, 2016 at Ursinus College.

Project Description

We examined mRNA expression of the 19 wnt proteins following acute skeletal muscle injury in mice on day 7 post-injury using PCR. A staining procedure for macrophages, endothelial cells, satellite cells, and fibroblasts was performed to observe which cell types were responsive to wnt signaling. This paper also includes a study of an optimal freezing protocol for skeletal muscle tissue for cryostat sectioning and histological examination.

Recommended Citation

Keoonela, Samantha and Garcia, Bradley, "Influence of Wnt Proteins Following Acute Skeletal Muscle Injury in Mice and Freezing Protocols for Skeletal Muscle Histology" (2016). Health and Exercise Physiology Summer Fellows. 4.
https://digitalcommons.ursinus.edu/health_sum/4