Submission Date

7-21-2023

Document Type

Paper- Restricted to Campus Access

Department

Health & Exercise Physiology

Faculty Mentor

Stephen C. Kolwicz Jr.

Student Contributor

Erica Locke

Second Student Contributor

Paige Ponzo

Comments

Presented during the 25th Annual Summer Fellows Symposium, July 21, 2023 at Ursinus College.

Project Description

Insulin is a hormone responsible for regulating glucose levels in the blood. Sensitivity to insulin has been shown to increase after exercise, resulting in less insulin needed to reduce glucose levels. Our previous findings indicated an increased sensitivity to insulin immediately after one bout of exercise in male mice, while females showed no change in sensitivity. Additionally, sedentary female mice had an increase in insulin sensitivity compared to male sedentary mice. However, the persistence of improved insulin sensitivity after one bout of acute exercise is unclear. Our study examined insulin sensitivity 24 hours after a bout of acute exercise. Since diurnal variation occurs in blood glucose levels, we performed tests in both the morning and afternoon. Male (n=17) and female (n=18) mice were assigned to a sedentary and exercise group and tested in the morning or afternoon. The exercise group ran on a motorized treadmill at 15 m/min for one hour. After 24 hours, mice were fasted for 90 minutes and then injection with one unit of insulin. Blood glucose levels were measured at 15, 30-, 60-, 90-, and 120-minute intervals using a handheld glucometer. Insulin sensitivity was quantified using an area under the curve analysis. Tissues were harvested to analyze the protein expression of glucose transporter 4. Findings from the study will provide insight to the effectiveness of acute exercise on insulin sensitivity.

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