Differences in Endothelial Permeability Following Injection of 25 Microliters of Barium Chloride vs. 10 Milligrams per Gram of Body Weight at the Tibialis Anterior Muscle of 4 and 12 Week-Old Mice

Author

James McDaid, Ursinus College

Submission Date

7-19-2019

Document Type

Paper- Restricted to Campus Access

Department

Health & Exercise Physiology

Faculty Mentor

April Carpenter

Comments

Presented during the 21st Annual Summer Fellows Symposium, July 19, 2019 at Ursinus College.

Project Description

Following skeletal muscle injury, the body’s response is inflammation of the affected area, with increased endothelial permeability to allow the entry of macrophages for healing. Using mice, we injected barium chloride into one of the tibialis anterior muscles to chemically injure the muscle, and sodium chloride into the other as a control. Evan’s Blue dye was injected into the bloodstream, which then attached to the protein albumin. Because albumin is too large to leave the blood stream, entry of albumin into the muscle indicates elevated permeability. Previously, we have injected 25 microliters of BaCl₂ and NaCl regardless of weight, but here, we injured half of the mice with 10 milligrams per gram of body weight, and compared these results to our previously used method. After removing the muscles, we weighed them, measured absorbance of the blue dye, and compared results based on muscle weight. Four and twelve week old males and females were used in this experiment.

Recommended Citation

McDaid, James, "Differences in Endothelial Permeability Following Injection of 25 Microliters of Barium Chloride vs. 10 Milligrams per Gram of Body Weight at the Tibialis Anterior Muscle of 4 and 12 Week-Old Mice" (2019). Health and Exercise Physiology Summer Fellows. 16.
https://digitalcommons.ursinus.edu/health_sum/16