Submission Date

4-27-2015

Document Type

Paper- Restricted to Campus Access

Department

Chemistry

Adviser

Eric Williamsen

Committee Member

Eric Williamsen

Committee Member

Mark Ellison

Committee Member

Rebecca Lyczak

Department Chair

Mark Ellison

Project Description

High-Performance Liquid Chromatography (HPLC) is one of the most widely used separation techniques and represents a critical step in the process of drug discovery, medical diagnostics, and even the criminal justice system. HPLC relies upon the differing intermolecular forces between compounds with the stationary phase of the column to separate analytes in time. These interactions change depending upon the solvent, temperature, pH, and other factors. By altering these conditions, the amount of time a compound is retained in the column can be modified. Precisely how these environmental differences alter the intermolecular forces and compound retention is not yet well characterized. A deeper understanding of these forces will allow for better separations and analyte-selective separation techniques. We have obtained the retention times for 26 different analytes at eight temperatures, in seven different mobile phases, and four column types. A subset of these data has been reevaluated and expanded in order to confirm their validity. This subset was then analyzed using several techniques, including multivariate (cluster, principle component, etc.), k-k, and Linear Solvation Energy Relationship (LSER) analyses, to reveal trends and characterize the forces governing the separation process in fluoro octyl and fluoro phenyl stationary phases.

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