Submission Date
4-27-2015
Document Type
Paper- Restricted to Campus Access
Department
Chemistry
Adviser
Eric Williamsen
Committee Member
Eric Williamsen
Committee Member
Mark Ellison
Committee Member
Rebecca Lyczak
Department Chair
Mark Ellison
Project Description
High-Performance Liquid Chromatography (HPLC) is one of the most widely used separation techniques and represents a critical step in the process of drug discovery, medical diagnostics, and even the criminal justice system. HPLC relies upon the differing intermolecular forces between compounds with the stationary phase of the column to separate analytes in time. These interactions change depending upon the solvent, temperature, pH, and other factors. By altering these conditions, the amount of time a compound is retained in the column can be modified. Precisely how these environmental differences alter the intermolecular forces and compound retention is not yet well characterized. A deeper understanding of these forces will allow for better separations and analyte-selective separation techniques. We have obtained the retention times for 26 different analytes at eight temperatures, in seven different mobile phases, and four column types. A subset of these data has been reevaluated and expanded in order to confirm their validity. This subset was then analyzed using several techniques, including multivariate (cluster, principle component, etc.), k-k, and Linear Solvation Energy Relationship (LSER) analyses, to reveal trends and characterize the forces governing the separation process in fluoro octyl and fluoro phenyl stationary phases.
Recommended Citation
Willard, Peter A., "Statistical and Linear Solvation Retention Relationship (LSRR) Studies of Retention on Fluorinated and Non–Fluorinated High–Performance Liquid Chromatographic (HPLC) Stationary Phases" (2015). Chemistry Honors Papers. 2.
https://digitalcommons.ursinus.edu/chem_hon/2
