Isolation of Complement Components 3 and 4, and Interaction Studies With the Lectin-like Domain of Thrombomodulin

Author

Thomas E. Holt, Ursinus CollegeFollow

Submission Date

9-8-2016

Document Type

Paper- Restricted to Campus Access

Department

Biochemistry & Molecular Biology

Faculty Mentor

Julia Koeppe

Second Faculty Mentor

Eric Williamsen

Comments

Presented during the 18th Annual Summer Fellows Symposium, July 22, 2016 at Ursinus College.

Project Description

A method for the isolation of complement components 3 and 4 from bovine plasma is described, and a pull-down analysis of those proteins with the lectin-like domain of thrombomodulin is analyzed for the presence of an interaction. No definitive answers are reached regarding the presence of the interaction. Additional future studies using techniques with higher levels of sensitivity will be applied in continuing work on this project.

Recommended Citation

Holt, Thomas E., "Isolation of Complement Components 3 and 4, and Interaction Studies With the Lectin-like Domain of Thrombomodulin" (2016). Biochemistry and Molecular Biology Summer Fellows. 7.
https://digitalcommons.ursinus.edu/biochem_sum/7