Submission Date
4-26-2024
Document Type
Paper- Restricted to Campus Access
Department
Biochemistry & Molecular Biology
Adviser
Dale Cameron
Committee Member
Anthony Lobo
Committee Member
Denise Finney
Department Chair
Eric Williamsen
Department Chair
Anthony Lobo
Project Description
Proteins are essential, functionally diverse macromolecules found in all living cells. Their structure is integral to their function, and they can fold in a variety of different conformations, which results in a change or loss of function. A specific class of misfolded proteins, known as prions, can transmit their structure onto natively folded proteins of the same type. While they are not known to cause disease in Saccharomyces cerevisiae (yeast), prions and similar aggregation patterns have been linked to neurodegenerative diseases in other organisms. Prion disease-causing proteins, like mammalian PrPSC causing transmissible spongiform encephalopathies (TSEs), and proteins underlying prion-like disease states, like α-synuclein (Parkinson’s disease), amyloid-β (Alzheimer’s disease), and Huntingtin (Huntington’s disease) are some of the most well-known cases. Cells have developed machinery to promote correct protein folding, consisting primary of molecular chaperones. These proteins are varied throughout the cell in location and function. Some exist on the nascent polypeptide exit tunnel of ribosomes to ensure proteins fold correctly during synthesis. Two of these complexes are known as the ribosome-associated complex (RAC) and nascent chain-associated complex (NAC), have been shown in previous studies from the Cameron lab to individually play important roles in reducing prion formation and promoting survival in yeast experiencing stress. This study seeks to better understand the functional relationship between the RAC and NAC components in yeast by comparing prion formation and stressed cell survival between pairwise deletion strains lacking combinations of RAC and NAC subunits.
Recommended Citation
Wrinn, Caitlin, "Determining Functional Interactions Between Ribosomal Co-translational Chaperone Complexes RAC and NAC (Btt1) in Saccharomyces cerevisiae" (2024). Biochemistry and Molecular Biology Honors Papers. 19.
https://digitalcommons.ursinus.edu/biochem_hon/19