Submission Date


Document Type

Paper- Restricted to Campus Access




Jennifer King

Committee Member

Samantha Wilner

Committee Member

Lauren Makuch

Department Chair

Carlita Favero

Project Description

As the primary macrophage of the central nervous system, microglia play a significant role in the immune response of the brain. Studying microglia dysfunction is important for understanding neurodegenerative disease pathology, such as HIV-associated neurocognitive disorder (HAND). Immortalized cell lines or primary microglia from neonatal mice are the most commonly used and accessible in vitro microglia models because isolating and culturing cells from adult mice is much more difficult, timely, and costly. However, because of their natural ageing, primary adult microglia are a much more physiologically adequate model for neurodegenerative diseases. The aim of this project is to establish a method for successfully isolating these primary microglia from adult mice to be used in further studies examining their neuroinflammatory response and its role in neurodegeneration.