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High-Pressure Liquid Chromatography (HPLC) is a widely applicable purification technique with uses in the biochemical, medical, and forensic disciplines. The mixture is dissolved in a mobile phase which is pumped past the stationary phase. Each mixture component interacts with the stationary phase for a different amount of time causing varying travel times and allowing for the collection of individual components. The most prominent mode — the mode used here — is reversed phase HPLC, which relies on intermolecular interactions between the nonpolar stationary phase, more polar mobile phase, and the compounds to be separated. Although dispersion interactions are considered the most prominent, other intermolecular interactions are important to the separation, but the relative magnitude and type of these interactions are not well understood. By gaining a more complete understanding of these processes, an improved method of RP-HPLC can be achieved by reducing time, cost, and environmental impact.
Kritz, Danielle A., "Characterization of a Phenyl High-Performance Liquid Chromatographic Stationary Phase" (2015). Chemistry Summer Fellows. 7.
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