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Health & Exercise Physiology
Macrophages produce growth factors that stimulate specific pathways to aid in skeletal muscle repair. Increasing permeability of endothelial cells post-injury allows for important cells and nutrients to migrate to the affected area. Certain macrophage-derived products aid in muscular repair by increasing endothelial stress fiber formation and thus increasing permeability. We use two genetically modified lines of mice: one line has macrophages that are unable to induce wnt signaling, while the other line releases wnt proteins normally. We isolated macrophage derived supernatant from both lines of mice and introduced them to HMEC cultures to induce stress fiber formation. Using florescent microscopy, we are able to visualize the difference in stress fiber formation between both lines of mice.
Salik, Oniel, "Effects of Macrophage Produced Wnts on Endothelial Permeability and Stress Fiber Formation" (2018). Biology Summer Fellows. 66.