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In order for the single celled embryo of C. elegans to develop, it must have a proper anterior-posterior axis development. A gene that plays an important role in that development is pam-1 which codes for the puromycin sensitive aminopeptidase (PAM-1) protein. In order to determine the unknown targets, we study the suppressors of pam-1. In this experiment we specifically studied suppressors, lz6 and lz4. Our goal is to determine the chromosomal location of lz6 and lz4 by using techniques such as SNP mapping, PCR, and DNA sequencing. Mutated pam-1 also delays meiotic exit, so we plan to observe if suppressors can rescue that process too. We have measured the wild type meiotic timings as a basis to compare with the pam-1 and suppressed strains. We have also mapped general areas of each chromosome for lz6, and we have ruled out the three candidate genes of lz4.
Hong, Angela, "Identifying Suppressor Mutations of the pam-1 Gene in C. elegans" (2018). Biology Summer Fellows. 58.