Currently, we are working with two genes: pam-1, which functions in the asymmetric division in meiosis and corresponding polarity, and wee-1.3, which functions in oocyte maturation. The mutation pam-1 is known to disturb embryo viability and reduces the hatch rate to 2% in the worms. However, a rescue mutation, wee-1.3, is known to bring the hatch rate up to 42% because polarity and asymmetry defects are suppressed. From previous research we know that during meiosis, the pam-1 mutation results in meiotic exit delays and other mutant phenotypes such as failure of meiosis II. Currently, we are imaging and analyzing strain US161, the strain of worm that has both mutations in its genotype, to see if the wee-1.3 mutation rescues the pam-1 meiosis phenotypic mutations as it does for the hatch rate. We look for particular time points in each series of images: the first polar body, the second polar body, and the pronucleus.
Lear, Sophie and Bender, Alex, "Investigating the Effect of the wee-1.3 and pam-1 Mutations on Meiosis After the Fertilization of Oocytes in C. elegans" (2021). Biology Presentations. 22.
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