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Biochemistry & Molecular Biology
The unique structure-function relationship of proteins has made de novo metalloprotein models a valuable tool in obtaining a deeper understanding of these essential biological molecules. To study the impact of active site configuration on reactivity, we use a de novo four helix bundle protein (DFsc) containing a non-heme di-iron center in which the active site has been varied. These variants were overexpressed in E. coli cells and purified via HPLC. The purified protein variants were then characterized through differing methods: (1) chromogenic assays to measure the reactivity of the proteins, (2) cyclic voltammetry to investigate their electrochemical properties, and (3) X-ray crystallography to determine the specific structure of the variants. This understanding of the protein structure will provide insight on how the different active site variations affect midpoint potentials and ultimately its reactivity.
Hawkins, Stephanie, "Characterization of De Novo Di-Iron Metalloproteins" (2018). Biochemistry and Molecular Biology Summer Fellows. 11.