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Biochemistry & Molecular Biology
This paper has met the requirements for Distinguished Honors
This study seeks to understand the origins and consequences of prions in baker's yeast, Saccharomyces cerevisiae. Specifically, we aim to demonstrate that prions can misfold not only post-translationally, but also during their synthesis on ribosomes as well. To that end, I have developed a variety of biochemical tools to assess the aggregation profile of three prion proteins (Sup35, Rnq1, and Ure2) on ribosomes. Additionally, a distinct yet related project explores one prion, [PSI+], and its effects on the entire yeast proteome. We aim to understand how prions can modulate gene expression at the translational level, as well as how prion-dependent changes in protein expression are shaped by the presence of environmental stressors.
Allwein, Benjamin T., "Prion Protein Aggregation in Yeast: Molecular and Cellular Implications" (2018). Biochemistry and Molecular Biology Honors Papers. 5.