Submission Date

4-23-2018

Document Type

Paper- Restricted to Campus Access

Department

Biochemistry & Molecular Biology

Adviser

Dale Cameron

Committee Member

Rebecca Roberts

Committee Member

Christian Rice

Committee Member

Dale Cameron

Department Chair

Anthony Lobo

Department Chair

Eric Williamsen

External Reviewer

Justin Hines

Distinguished Honors

This paper has met the requirements for Distinguished Honors

Project Description

This study seeks to understand the origins and consequences of prions in baker's yeast, Saccharomyces cerevisiae. Specifically, we aim to demonstrate that prions can misfold not only post-translationally, but also during their synthesis on ribosomes as well. To that end, I have developed a variety of biochemical tools to assess the aggregation profile of three prion proteins (Sup35, Rnq1, and Ure2) on ribosomes. Additionally, a distinct yet related project explores one prion, [PSI+], and its effects on the entire yeast proteome. We aim to understand how prions can modulate gene expression at the translational level, as well as how prion-dependent changes in protein expression are shaped by the presence of environmental stressors.

Comments

Supported by a NIH Academic Research Enhancement Award (AREA) Grant R15GM119081-01.

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