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Health & Exercise Physiology
We examined mRNA expression of the 19 wnt proteins following acute skeletal muscle injury in mice on day 7 post-injury using PCR. A staining procedure for macrophages, endothelial cells, satellite cells, and fibroblasts was performed to observe which cell types were responsive to wnt signaling. This paper also includes a study of an optimal freezing protocol for skeletal muscle tissue for cryostat sectioning and histological examination.
Keoonela, Samantha and Garcia, Bradley, "Influence of Wnt Proteins Following Acute Skeletal Muscle Injury in Mice and Freezing Protocols for Skeletal Muscle Histology" (2016). Health and Exercise Physiology Summer Fellows. Paper 4.